製品の概要

  • 製品名Anti-Cardiotrophin 1 antibody [3G6D9]
    Cardiotrophin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3G6D9] to Cardiotrophin 1
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant full length protein (Human). (N-terminal HisTag, 22.5 kDa protein, 200 aa residues of the human cardiotrophin and 12 additional aa residues).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
  • 組織特異性Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
  • 配列類似性Belongs to the IL-6 superfamily.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiophin 1 antibody
    • Cardiophin1 antibody
    • Cardiotrophin I antibody
    • Cardiotrophin-1 antibody
    • Cardiotrophin1 antibody
    • CardiotrophinI antibody
    • CT 1 antibody
    • CT-1 antibody
    • CT1 antibody
    • CTF 1 antibody
    • Ctf1 antibody
    • CTF1_HUMAN antibody
    see all

Anti-Cardiotrophin 1 antibody [3G6D9] 画像

  • IHC image of ab13975 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13975, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cardiotrophin 1 antibody [3G6D9] (ab13975) 使用論文

This product has been referenced in:
  • Nogueira-Silva C  et al. The role of glycoprotein 130 family of cytokines in fetal rat lung development. PLoS One 8:e67607 (2013). Read more (PubMed: 23826327) »
  • Natal C  et al. Cardiotrophin-1 is expressed in adipose tissue and upregulated in the metabolic syndrome. Am J Physiol Endocrinol Metab 294:E52-60 (2008). Read more (PubMed: 17940213) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"