製品の概要

  • 製品名Anti-Cardiotin antibody [R2G]
  • 製品の詳細
    Mouse monoclonal [R2G] to Cardiotin
  • 特異性This antibody reacts with cardiotin, a mitochondrion-associated protein which is present in cardiomyocytes and skeletal muscle.
  • アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Goat, Hamster, Cat, Dog, Human, Pig, Xenopus laevis, Monkey, Zebrafish
  • 免疫原

    Total protein extract of chicken gizzard

  • 特記事項Mitochondrial membrane proteins have been shown to be involved in several cellular processes such as the regulation of apoptosis and the energy metabolism. Cardiotin antibodies, which are reactive with mitochondrial proteins, have proven to be early indicators of ischemia induced changes in the myocardium.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/25 - 1/500.

ターゲット情報

  • 関連性Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondrial membrane. The cardiotin structure exists as subunits of 60 kDa and 100 kDa. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. This monoclonal antibody can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.

Anti-Cardiotin antibody [R2G] 画像

  • Human atrial cardiomyocytes stained with the monoclonal cardiotin antibody R2G.

  • Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.
  • Immunofluorescence staining of a 7 days old zebrafish embryo

Anti-Cardiotin antibody [R2G] (ab8962) 使用論文

This product has been referenced in:
  • Talavera-Adame D  et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). Read more (PubMed: 19364272) »
  • Dispersyn GD  et al. Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones. Eur Heart J 23:849-57 (2002). Read more (PubMed: 12042006) »

See all 2 Publications for this product

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