Anti-Cardiac Troponin T 抗体 (ab89221)

製品の概要

  • 製品名Anti-Cardiac Troponin T antibody
    Cardiac Troponin T 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Cardiac Troponin T
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-255 of human Cardiac Troponin T (AAH02653) linked to a proprietary tag.

  • ポジティブ・コントロール
    • COLO 320 HSR cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.
    sELISA: Use at a concentration of 10 µg/ml. The detection limit for recombinant tagged Cardiac Troponin T is approximately 1 ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • 組織特異性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
    • 関連疾患Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    • 配列類似性Belongs to the troponin T family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    Anti-Cardiac Troponin T antibody 画像

    • Anti-Cardiac Troponin T antibody (ab89221) at 5 µg/ml + immunogen at 0.2 µg
      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : ~65 kDa (why is the actual band size different from the predicted?)
    • Anti-Cardiac Troponin T antibody (ab89221) at 5 µg/ml + COLO 320 HSR cell lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : ~45 kDa. We are unsure as to the identity of these extra bands.

    Anti-Cardiac Troponin T antibody (ab89221) 使用論文

    ab89221 has not yet been referenced specifically in any publications.

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    The antibody is raised against a recombinant fragment corresponding to amino acids 1-255 of human Cardiac Troponin T (AAH02653) linked to a proprietary tag. The epitope that the monoclonal antibody recognizes has...

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