製品の概要

  • 製品名Anti-Cardiac Troponin T antibody
    Cardiac Troponin T 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Cardiac Troponin T
  • アプリケーション適用あり: IP, ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human cardiac troponin T, purity more than 98%

  • ポジティブ・コントロール
    • IHC-P: FFPE human heart tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 機能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 組織特異性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 関連疾患Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 配列類似性Belongs to the troponin T family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody 画像

  • IHC image of Cardiac Troponin T  staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64623, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of transverse cryosections of uninjured newt heart tissue (ventricle), staining Cardiac Troponin T with ab64623.

    Sections were fixed with 4% formaldehyde and blocked with 10% swine serum. Samples were incubated with primary antibody overnight at 4°C and an AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.

Anti-Cardiac Troponin T antibody (ab64623) 使用論文

This product has been referenced in:
  • Witman N  et al. miR-128 regulates non-myocyte hyperplasia, deposition of extracellular matrix and Islet1 expression during newt cardiac regeneration. Dev Biol 383:253-63 (2013). Read more (PubMed: 24055866) »
  • Ikonen L  et al. 2D and 3D self-assembling nanofiber hydrogels for cardiomyocyte culture. Biomed Res Int 2013:285678 (2013). ICC/IF ; Rat . Read more (PubMed: 23573513) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Pig Tissue sections (Heart)
Specification Heart
Permeabilization Yes - 0,3% Triton
Fixative Paraformaldehyde
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投稿 Feb 03 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"