製品の概要
- 製品名Anti-Cardiac Troponin T antibody [9G6] (HRP)
Cardiac Troponin T 一次抗体 製品一覧 - 製品の詳細Mouse monoclonal [9G6] to Cardiac Troponin T (HRP)
- 標識HRP
- アプリケーション適用あり: IHC-P, WB, ELISA, Other, Sandwich ELISAmore details
- 種交差性交差種: Human
- 免疫原
Full length native protein (purified) (Human).
- エピトープ1-60 aa
- ポジティブ･コントロール
- IHC-P: Human heart FFPE tissue sections.
- 特記事項
This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, or TnC or cTnI.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C.
- バッファーPreservative: 0.05% Proclin
Constituents: PBS, pH 7.4 -
Concentration information loading... - 精製度Protein A purified
- 一次抗体 備考This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, or TnC or cTnI.
- ポリ/モノモノクローナル
- クローン名9G6
- ミエローマSp2/0
- アイソタイプIgG1
- 軽鎖の種類unknown
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
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- 450 nm Stop Solution for TMB Substrate (ab171529)
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- Immunoassay Blocking Buffer (ab171534)
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- sELISA pair antibody
アプリケーション
Our Abpromise guarantee covers the use of ab8296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| Other | Use at an assay dependent concentration. | |
| Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1C11] to Cardiac Troponin T (ab8295). Can be used as Detection antibody. |
ターゲット情報
- 機能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
- 組織特異性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
- 関連疾患Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. - 配列類似性Belongs to the troponin T family.
- Information by UniProt
-
参照データベース
- Entrez Gene: 7139 Human
- Omim: 191045 Human
- SwissProt: P45379 Human
- Unigene: 533613 Human
-
別名
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Anti-Cardiac Troponin T antibody [9G6] (HRP) 画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296)](http://a.abcam.com/ps/products/8/ab8296/Images/ab8296-172913-ab8296heartmuscleugmled.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296)IHC image of Cardian Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8296, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296) 使用論文
ab8296 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"