Anti-Cardiac Troponin T 抗体 [9G6] (HRP) (ab8296)

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製品の概要

  • 製品名
    Anti-Cardiac Troponin T antibody [9G6] (HRP)
    Cardiac Troponin T 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9G6] to Cardiac Troponin T (HRP)
  • 由来種
    Mouse
  • 標識
    HRP
  • アプリケーション
    適用あり: IHC-P, WB, ELISA, Other, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human).

  • エピトープ
    1-60 aa
  • ポジティブ･コントロール
    • IHC-P: Human heart FFPE tissue sections.
  • 特記事項


    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, or TnC or cTnI.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1C11] to Cardiac Troponin T (ab8295). Can be used as Detection antibody.

ターゲット情報

  • 機能
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 組織特異性
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 関連疾患
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 配列類似性
    Belongs to the troponin T family.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296)

      IHC image of Cardian Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8296, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献

    ab8296 has not yet been referenced specifically in any publications.

    ab8296 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    5 reviews or Q&A

    10
    Votes: Highest First

    cardiac troponin ELISA antibodies with protein for troponin T , I or C

    Thank you very much for your patience while I was investigating.


    I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Trop...

    Read More

    Respected Sir,
    Many thanks for your response regarding my queries, I can purchase the product but can you please check once the all three combination can work in the format as below. Please let me know that how much discount you are offering to gi...

    Read More

    Thank you for your email.

    The two antibodies will work together. The protein we do not know whether it will work in ELISA.

    This is why we could give you a testing discount on the protein. This means, you would buy the protein to the...

    Read More

    Hello Sir,

    I wish to purchase following items from web cam so can you please suggest me that the is that the right combination for ELISA?

    ab9937 Cardiac Troponin T Protein (AbCam Uk) 1Pack

    ab8295 Anti-Cardiac Troponin T...

    Read More

    Thank you very much for your interest in our products.

    I am happy to confirm that the ab8295 and the ab8296 are perfectly well choosen together. They will work as a sandwich pair in ELISA.

    The ab9937, which you would use as a standa...

    Read More

    Good Morning,
    I use these reagents for the detection of troponin.
    ab8295 mouse monoclonal (1C11) to cardiac Troponin T
    ab8296 mouse monoclonal (9G6) to cardiac Troponin T (HRP)
    ab9937 Cardiac Troponin T protein
    These reagents a...

    Read More

    Thank you for contacting us.

    Troponin T is a part of the troponin complex, essential for muscle contaction in skeletal and cardial muscle, but not smooth muscle.

    This antibody is specific to heart muscle, and does not cross react wi...

    Read More

    I selected the 'ab8295' because I found it can be reacted with rat (SD rat is our sample targert). However, after reading the datasheet of another 'ab8295' paired antibody "ab8296' which you recommanded. I found it is just reactive with human sample. T...

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    Thank you for your email. Another suggestion is pairing ab10220 (Mouse monoclonal [6G9] to cardiac Troponin T (HRP)) with ab8295. Please take a look at the online datasheet to see if this antibody would suit your needs. If you have any additional qu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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