Anti-Cardiac Troponin T 抗体 [1F11] (ab10214)

製品の概要

  • 製品名
    Anti-Cardiac Troponin T antibody [1F11]
    Cardiac Troponin T 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1F11] to Cardiac Troponin T
  • 特異性
    Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)
  • アプリケーション
    適用あり: Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, Other, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Fish
  • 免疫原

    Free human cardiac troponin T

  • エピトープ
    Peptide 146-160.
  • 特記事項
    Concentration varies from lot to lot and can be provided on request.


    Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 21278734

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details).
Other Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23066088

ターゲット情報

  • 機能
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 組織特異性
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 関連疾患
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 配列類似性
    Belongs to the troponin T family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody [1F11] 画像

  • Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
  • ab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).

    This image is courtesy of an Abreview submitted by Yiqiang Zhang.

    See Abreview

Anti-Cardiac Troponin T antibody [1F11] (ab10214) 使用論文

This product has been referenced in:
  • Jackson R  et al. Isolation of human explant derived cardiac stem cells from cryopreserved heart tissue. PLoS One 12:e0176000 (2017). Flow Cyt ; Human . Read more (PubMed: 28414815) »
  • De Pauw A  et al. Dnmt3a-mediated inhibition of Wnt in cardiac progenitor cells improves differentiation and remote remodeling after infarction. JCI Insight 2:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 28614798) »

See all 27 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (spontaneous differentiating iPSC)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
spontaneous differentiating iPSC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Cardiac cells)
Total protein in input
50 µg
Immuno-precipitation step
Protein A/G
Specification
Cardiac cells
Username

Abcam user community

Verified customer

投稿 Jun 27 2015

Thank you for your contact. I am very sorry to hear that the vial was empty.
This is to let you know that I have placed a new order as a free of charge replacement of ab10214. The new order number is 1217998.
If you need any further assistanc...

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Thank you for contacting us.
I am sorry to hear that the antibody that worked previously is not working anymore.

I checked our records for this lot number, but have not found any complaints. We do not test any antibodies in SPR, thus in ge...

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Thank you for contacting us and for providing this image.

After review of the image provided, we believe that the extra bands which you are seeing are result of non-specific background. This will show up when using too much protein, too mu...

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I could certainly offer you a code number. Would you be so kind to confirm the name and the address of your research institute? We need this information only for our internal record.

Thank you and I look forward to hearing from you ...

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Thank you for your enquiry and your interest in our products.

I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal probl...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Tissue lysate - whole (heart tissue)
Loading amount
20 µg
Specification
heart tissue
Gel Running Conditions
Reduced Denaturing (13)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (ES cell EBs differentiated for 12 days.)
Specification
ES cell EBs differentiated for 12 days.
Fixative
Methanol
Permeabilization
Yes - 0.1%triton-x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (cardiomyocytes)
Specification
cardiomyocytes
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Username

Dr. Yiqiang Zhang

Verified customer

投稿 Jun 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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