Anti-Cardiac Troponin I 抗体 [EP1106Y] (ab52862)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 18436862
WB 1/100000 - 1/1e+006. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/50.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/250 - 1/500.

ターゲット情報

  • 機能Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 関連疾患Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性Belongs to the troponin I family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Anti-Cardiac Troponin I antibody [EP1106Y] 画像

  • IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52862, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862) at 1/1000000 dilution + fetal heart cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • ab52862 at 1/250 dilution staining cardiac Troponin I in human cardiac muscle by Immunohistochemistry, Paraffin embedded tissue.

Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862) 使用論文

This product has been referenced in:
  • Seki T  et al. Generation and characterization of functional cardiomyocytes derived from human T cell-derived induced pluripotent stem cells. PLoS One 9:e85645 (2014). ICC/IF ; Human . Read more (PubMed: 24465630) »
  • Liang D  et al. miR-10a regulates proliferation of human cardiomyocyte progenitor cells by targeting GATA6. PLoS One 9:e103097 (2014). ICC/IF ; Human . Read more (PubMed: 25068583) »

See all 7 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Cardiac)
Permeabilization Yes - 0.05% TritionX100
Specification Cardiac
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Sep 16 2016

Thank you for contacting us.

The concentration of Rabbit mAB against cardiac troponin I (EP1106Y - 100 microL - lot GR36789-4) is xxxxxxxx mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you...

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I can confirm that ab52862 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated o...

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I am very pleased to hear you would like to accept our offer and test ab52862 in Flow Cytometry. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem ...

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Thank you for your reply. I am currently in contact with the source of this monoclonal isotype control antibody as due to licensing issues it indeed seems difficult to get it, and I am awaiting a reply. I will forward to you the information as soon as ...

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Thank you for your enquiry. If you have a polyclonal rabbit IgG isotype control already at hand, you could use it for this rabbit monoclonal, as amongst other isotypes it may also include the isotype of the monoclonal antibody and therefore resemble...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Cardiomyocytes)
Specification Cardiomyocytes
Fixative Methanol
Permeabilization Yes - 0.2% Triton-x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 May 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"