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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human cardiac Troponin I.
(Peptide available as ab47002.)
Our Abpromise guarantee covers the use of ab47003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Cardiac Troponin I peptide (ab47002).|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21909276
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
This image is courtesy of an Abreview submitted by Dr. Mario TorradoBlocking Step: 20% Serum for 3 hours at 24°C
Immunohistochemical analysis of frozen mouse heart tissue labeling Cardiac Troponin I with ab47003 at 1/100 dilution.