製品の概要

  • 製品名Anti-Cardiac Troponin I antibody
    Cardiac Troponin I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cardiac Troponin I
  • アプリケーション適用あり: WB, ICC/IF, IHC-Fr, IHC-P, Flow Cyt, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human cardiac Troponin I.

    (Peptide available as ab47002.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Heart (human) tissue lysate, mouse heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Cardiac Troponin I peptide (ab47002).
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/100.
IHC-P 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 21909276

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 関連疾患Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性Belongs to the troponin I family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Anti-Cardiac Troponin I antibody 画像

  • All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab74003 staining Cardiac Troponin I in neonatal Rat cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400 in 3% BSA + PBS-T) for 12 hours at 4°C. An AlexaFluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution

    Lane 1 : Pig Heart whole cell lysate
    Lane 2 : Pig Heart whole cell lysate
    Lane 3 : Pig Heart whole cell lysate
    Lane 4 : Pig Heart whole cell lysate

    Lysates/proteins at 4 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Dr. Mario Torrado

    Blocking Step: 20% Serum for 3 hours at 24°C

    See Abreview

  • Immunohistochemical analysis of frozen mouse heart tissue labeling Cardiac Troponin I with ab47003 at 1/100 dilution.

    See Abreview

  • ab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-Cardiac Troponin I antibody (ab47003) 使用論文

This product has been referenced in:
  • Torrado M  et al. Pitx2c is reactivated in the failing myocardium and stimulates myf5 expression in cultured cardiomyocytes. PLoS One 9:e90561 (2014). Read more (PubMed: 24595098) »
  • Gilsbach R  et al. Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease. Nat Commun 5:5288 (2014). Read more (PubMed: 25335909) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (immobilization on beads)
Specification immobilization on beads
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Type Sandwich (Capture)
Username

Mr. Chang Su Jeon

Verified customer

投稿 Sep 19 2012

Application Immunocytochemistry
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Human Cultured Cells (Fetal Cardiomyocytes)
Specification Fetal Cardiomyocytes
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
Username

Jennifer Dewing

Verified customer

投稿 Mar 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Mar 22 2013

Thank you for contacting us.
I appreciate the time taken to submit protocol information. The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would also appreciate if you could please...

Read More

Thank you for getting back to me with the new results obtained.

It seems from the image shared that the staining is much improved with the changes in the protocol. The nuclear staining is much improved and the striations observed would be exp...

Read More

Thank you for taking time to complete our questionnaire and sorry for the delay in getting back to you. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult heart)
Specification Adult heart
Fixative Formaldehyde
Permeabilization Yes - 0.5% Tx100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (neonatal cardiomyocyte)
Specification neonatal cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mrs. Jamie Soto

Verified customer

投稿 Jun 15 2012

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"