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Human cardiac troponin C.
No cross reactivity with cTnT and cTnI. Will reconize TnC in binary complax with cTnI.
Our Abpromise guarantee covers the use of ab10245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.|
|Other||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab10245 staining Cardiac Troponin C in Human sternal bone marrow derived MSCs induced to cardio by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 10% serum for 1 hour at 37°C. Samples were incubated with primary antibody (1/250 in PBS + 1% Goat serum) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
ab10245 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"