Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiac myocytes)
Loading amount
15000 cells
Specification
Cardiac myocytes
Treatment
20uM phenylephrine 20 min 37C
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA/TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 30 kDa
Positive control
20uM phenylephrine 20min 37C
Negative control
1uM isoproterenol 5 min 37C
Additional data
Additional Notes
This produced a band of 30 kDa on a 15% SDS-PAGE Bio-Rad Criterion gel
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 25 2008