Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiac myocytes)
Loading amount
15000 cells
Specification
Cardiac myocytes
Treatment
20uM phenylephrine
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Positive control
20uM phenylephrine-treated cardiac myocytes
Negative control
1uM isoproterenol-treated cardiac myocytes
Additional data
Additional Notes
This antibody produced a band of 45kDa, which is the wrong size.
Troponin I is a protein of MW ~26kDa, and phospho-cTnI migrates around 29-30kDa in a 10% or 15% SDS-PAGE.
Troponin I is a protein of MW ~26kDa, and phospho-cTnI migrates around 29-30kDa in a 10% or 15% SDS-PAGE.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 09 2008