製品の概要

  • 製品名Anti-CARD9 antibody [EPR6488]
    CARD9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6488] to CARD9
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • THP1, Raji, Jurkat, and PC3 cell lysates; Human brain and liver tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値) KD = 1.79 x 10 -10 M Learn more about KD
  • バッファーpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR6488
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能Activates NF-kappa-B via BCL10.
    • 組織特異性Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
    • 関連疾患Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
    • 配列類似性Contains 1 CARD domain.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CANDF2 antibody
      • CARD9 antibody
      • CARD9_HUMAN antibody
      • Caspase recruitment domain family member 9 antibody
      • Caspase recruitment domain-containing protein 9 antibody
      • hCARD9 antibody
      see all

    Anti-CARD9 antibody [EPR6488] 画像

    • All lanes : Anti-CARD9 antibody [EPR6488] (ab124922) at 1/1000 dilution

      Lane 1 : THP1 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • ab124922, at 1/250 dilution, staining CARD9 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab124922, at 1/250 dilution, staining CARD9 in paraffin-embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CARD9 antibody [EPR6488] (ab124922) 使用論文

    ab124922 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124922.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"