製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CARD8
  • 特異性
    ab24186 reacts with CARD8 (CARDINAL/TUCAN) protein.
  • アプリケーション
    適用あり: ICC, Flow Cyt, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to amino acids within the last 50 amino acids of the C terminus of human CARD8, GenBank accession AAG50014

  • ポジティブ・コントロール
    • K562 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide.
    Constituents: PBS.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).

ターゲット情報

  • 機能
    Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
  • 組織特異性
    High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.
  • 配列類似性
    Contains 1 CARD domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptotic protein NDPP 1 antibody
    • Apoptotic protein NDPP1 antibody
    • CARD 8 antibody
    • CARD inhibitor of NF kappa B activating ligand antibody
    • CARD inhibitor of NF kappaB activating ligand antibody
    • CARD inhibitor of NF kappaB activating ligands antibody
    • CARD-inhibitor of NF-kappa-B-activating ligand antibody
    • CARD8 antibody
    • CARD8_HUMAN antibody
    • CARDINAL antibody
    • Caspase recruitment domain containing protein 8 antibody
    • Caspase recruitment domain family member 8 antibody
    • Caspase recruitment domain protein 8 antibody
    • Caspase recruitment domain-containing protein 8 antibody
    • DACAR antibody
    • Dakar antibody
    • DKFZp779L0366 antibody
    • FLJ18119 antibody
    • FLJ18121 antibody
    • KIAA0955 antibody
    • MGC57162 antibody
    • NDPP 1 antibody
    • NDPP antibody
    • NDPP1 antibody
    • TUCAN antibody
    • Tumor up regulated CARD containing antagonist of CASP9 antibody
    • Tumor up regulated CARD containing antagonist of caspase 9 antibody
    • Tumor up-regulated CARD-containing antagonist of CASP9 antibody
    • tumor up-regulated CARD-containing antagonist of caspase nine antibody
    • Tumor upregulated CARD containing antagonist of CASP9 antibody
    see all

画像

  • Lane 1 : Anti-CARD8 antibody (ab24186) at 2 µg/ml (CARD8 expression in K562 cell lysate)
    Lane 2 : Anti-CARD8 antibody (ab24186) at 4 µg/ml (CARD8 expression in K562 cell lysate)

    Lane 1 : As above
    Lane 2 : As above


    Predicted band size : 49 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab24186 at 10µg/ml staining CARD8 in K562 cells by ICC/IF

  • ab24186 staining CARD8 in human monocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab24186 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

  • ab24186 staining CARD8 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
    WGA = Wheat Germ Agglutinin

    See Abreview

参考文献

This product has been referenced in:
  • D'Osualdo A  et al. CARD8 and NLRP1 undergo autoproteolytic processing through a ZU5-like domain. PLoS One 6:e27396 (2011). WB ; Human . Read more (PubMed: 22087307) »
  • Mille F  et al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB ; Human . Read more (PubMed: 19465923) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (White Blood Cells (Mixed Population))
Loading amount
25 µg
Specification
White Blood Cells (Mixed Population)
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Mononuclear Cell Preparations)
Specification
Mononuclear Cell Preparations
Preparation
Cell harvesting/tissue preparation method: Apheresis
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Monocytes Lymphocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Mar 31 2011

Thank you for your enquiry. I have enquired at the originator of the product for the information that you requested. Unfortunately, the exact immunogen sequence is proprietary and can not be released. However, they did confirm that it is within the...

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Thank you for your email. The originator of this antibody has told me that they did a peptide blocking study and the only bands that disappeared were around 45kDa. The other bands are thought to be non-specific, but more testing to confirm or refute th...

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Thank you for your enquiry. The immunogen is proprietary information - it was a synthetic peptide corresponding to amino acids at the C terminus. However, the antibody will not cross-react with the CARD8 variant and should not cross-react with any oth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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