The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: peptide ELISA (antibody detection limit dilution >1 / 32000).
IHC-P: Use at a concentration of 5 µg/ml.
WB: Approx. 45-50 kDa and 25-30 kDa bands observed in A549, Human Heart and Human Muscle lysates at 1ug of primary. Please note that the band we observe is different from the predicted size of approx. 119 kDa according to NP_115976.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ab4381 at 5ug/ml staining CARD6 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.