Human Liver and Brain cerebellum tissues; A431 and Raji whole cell lysates.
The pattern of staining with this antibody is similar to that observed with ab189167 and ab115471 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver
Antigen retrieval conditions: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minute
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Immunohistochemistry of Formalin-fixed, Paraffin-embedded Kupffer cells in the sinusoids of the liver labeling CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.
Immunohistochemistry of Formalin-fixed, Paraffin-embedded Purkinje cells and fibers in the human cerebellum labeling CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.
Western blot - Anti-CARD11 antibody (ab113409)
All lanes : Anti-CARD11 antibody (ab113409) at 1/1000 dilution
Lane 1 : A431 whole cell lysate Lane 2 : Raji whole cell lysate