製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CARD10
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to 14 amino acids at the C terminus of human CARD10 (Genbank accession No.AAK26165).

  • ポジティブ・コントロール
    • EL4 cell lysate can be used as a positive control.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 116 kDa.Can be blocked with Human CARD10 peptide (ab39737).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Activates NF-kappa-B via BCL10 and IKK.
  • 組織特異性
    Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.
  • 配列類似性
    Contains 1 CARD domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bcl10 binding protein and activator of NFKB antibody
    • BIMP1 antibody
    • CAR10_HUMAN antibody
    • CARD 10 antibody
    • CARD containing MAGUK 3 protein antibody
    • CARD-containing MAGUK 3 antibody
    • CARD-containing MAGUK protein 3 antibody
    • CARD10 antibody
    • Carma 3 antibody
    • CARMA3 antibody
    • Caspase Recruitment Domain family member 10 antibody
    • Caspase recruitment domain-containing protein 10 antibody
    see all

Anti-CARD10 antibody 画像



  • Predicted band size : 116 kDa
    Western Blot of A20 cell lysate labeling CARD10 with Anti-CARD10 antibody (ab36839) at 5µg/ml.
  • Immunofluorescence of CARD10 in EL4 cells using ab36839 at 20 ug/ml.

Anti-CARD10 antibody (ab36839) 使用論文

This product has been referenced in:
  • Causton B  et al. CARMA3 Is Critical for the Initiation of Allergic Airway Inflammation. J Immunol 195:683-94 (2015). IF ; Mouse . Read more (PubMed: 26041536) »
  • Hicks SD & Miller MW Effects of ethanol on transforming growth factor ?1-dependent and -independent mechanisms of neural stem cell apoptosis. Exp Neurol 229:372-80 (2011). WB, ICC ; Mouse . Read more (PubMed: 21419121) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us regarding this. I could not find any historical data showing that we had offered an anti-Card10 or anti-CARM3 raised in sheep. I hope this information is helpful. Please let me know if have any questions.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate Buffer pH 6
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Buffered Formalin
Username

Abcam user community

Verified customer

投稿 Sep 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Sep 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6
Permeabilization
No
Specification
pancreatic tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

投稿 Sep 09 2015

Thank you for your enquiry. I have enquired at the originator of the product and they have confirmed that the immunogen for this antibody has no homology with CARD11 and a BLAST search does not yield proteins that large, so cross-reaction with line...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録