Anti-Carcino Embryonic Antigen CEA 抗体 [26/3/13] (ab4451)

製品の概要

  • 製品名
    Anti-Carcino Embryonic Antigen CEA antibody [26/3/13]
    Carcino Embryonic Antigen CEA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA
  • 特異性
    This antibody recognises exclusively CEACAM 5 transiently expressed on the cell surface of transfected BOSC cells. It can be used to distinguish CEACAM 5 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM7), CEACAM 6 (NCA/CD66c), CEACAM 7 (CGM2), CEACAM 8 (CGM6/CD66b)and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members. This antibody was included and characterized in the studies from the VIth Leucocyte Typing Workshop (Grunert F et al. 1994)
  • アプリケーション
    適用あり: IHC-P, Sandwich ELISA, ELISA, Flow Cyt, WB, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Grunert F, et al. 1985)

  • ポジティブ・コントロール
    • IHC-P: human normal colon tissue sections
  • 特記事項
    Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.2 µg/ml.

For sandwich ELISA, use this antibody as Capture at 0.2µg/ml

ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • 組織特異性
    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • 翻訳後修飾
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

画像

  • IHC image of Carcino Embryonic Antigen CEA staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4451, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Standard Curve for Carcino Embryonic Antigen CEA dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451) at 0.2ug/ml and Detector Antibody Rabbit polyclonal to Carcino Embryonic Antigen CEA (ab15987) at 0.5ug/ml

参考文献

This product has been referenced in:
  • Stern LA  et al. Geometry and expression enhance enrichment of functional yeast-displayed ligands via cell panning. Biotechnol Bioeng 113:2328-41 (2016). Read more (PubMed: 27144954) »
  • Zeng WJ  et al. Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification. Int J Nanomedicine 10:1759-68 (2015). IHC-P ; Human . Read more (PubMed: 25784803) »

See all 8 Publications for this product

レビューと Q&A

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Application
ELISA
Sample
Human Purified protein (not sure)
Specification
not sure
Type
Sandwich (Capture)
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投稿 Jan 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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