Anti-Carboxypeptidase Y 抗体 [10A5B5] (ab113685)

製品の概要

  • 製品名Anti-Carboxypeptidase Y antibody [10A5B5]
    Carboxypeptidase Y 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10A5B5] to Carboxypeptidase Y
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Full length native protein (purified)

  • ポジティブ・コントロール
    • Yeast membranes
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use a concentration of 20 µg/ml.

ターゲット情報

  • 機能Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
  • 配列類似性Belongs to the peptidase S10 family.
  • 翻訳後修飾Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).
  • 細胞内局在Vacuole. Lysosome-like vacuoles.
  • Information by UniProt
  • 参照データベース
    • 別名
      • carboxypeptidase C PRC1 antibody
      • Carboxypeptidase Y antibody
      • Carboxypeptidase YSCY antibody
      • CBPY_YEAST antibody
      • CPY antibody
      • LBC1 antibody
      • PRC1 antibody
      • Vacuolar carboxypeptidase Y antibody
      see all

    Anti-Carboxypeptidase Y antibody [10A5B5] 画像

    • All lanes : Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

      Lane 1 : Yeast whole cell lysate
      Lane 2 : Yeast membrane preparation

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 59 kDa

    Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685) 使用論文

    This product has been referenced in:
    • Ast T  et al. A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins. J Cell Sci 127:3017-23 (2014). WB ; Saccharomyces cerevisiae . Read more (PubMed: 24849653) »
    • Manandhar SP  et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). Read more (PubMed: 23166297) »

    See all 4 Publications for this product

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