製品の概要

  • 製品名Anti-Carboxypeptidase H antibody
    Carboxypeptidase H 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Carboxypeptidase H
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (GMRRRRRLQQ EDGISFEYHR YPELREALVS VWLQCTAISR IYTVGRSFEG) of Human Carboxypeptidase H (NP_001864).

  • ポジティブ・コントロール
    • MCF7 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
  • 組織特異性Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
  • 配列類似性Belongs to the peptidase M14 family.
  • 細胞内局在Nucleus and Secreted. Secretory granules of pancreatic islets, adrenal gland, pituitary and brain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Carboxypeptidase E antibody
    • Carboxypeptidase H antibody
    • Carboxypeptidase H precursor antibody
    • CarboxypeptidaseE antibody
    • CarboxypeptidaseH antibody
    • CBPE_HUMAN antibody
    • Cobalt stimulated chromaffin granule carboxypeptidase antibody
    • CPE antibody
    • Cph 1 antibody
    • CPH antibody
    • Enkephalin convertase antibody
    • Entephalin convertase antibody
    • Insulin granule associated carboxypeptidase antibody
    • Prohormone processing carboxypeptidase antibody
    • Prohormone-processing carboxypeptidase antibody
    see all

Anti-Carboxypeptidase H antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Carboxypeptidase H with ab84631 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Carboxypeptidase H. Right - Merge.
  • Anti-Carboxypeptidase H antibody (ab84631) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling Carboxypeptidase H with ab84631 at 5µg/ml.

Anti-Carboxypeptidase H antibody (ab84631) 使用論文

ab84631 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"