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N Epsilon (Carboxymethyl) Lysine (CML)-HSA.
Our Abpromise guarantee covers the use of ab30917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.1 - 0.5 µg/ml.|
|IHC-Fr||Use a concentration of 2 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
ab30917 staining Carboxymethyl Lysine in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and embedded in paraffin. Samples were incubated with primary antibody (1/125 in PBS + 1% BSA) for overnight at 4°C. A Biotin-conjugated secondary was used with DAB visualisation the samples were then counterstained with haematoxylin.