Anti-Carboxymethyl Lysine 抗体 [NF-1G] (ab30917)

製品の概要

  • 製品名Anti-Carboxymethyl Lysine antibody [NF-1G]
    Carboxymethyl Lysine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NF-1G] to Carboxymethyl Lysine
  • 特異性This antibody is suitable for the detection of N Epsilon (Carboxymethyl) Lysine (CML) in tissues and tissue extracts.
  • アプリケーション適用あり: ELISA, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    N Epsilon (Carboxymethyl) Lysine (CML)-HSA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • 別名
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

Anti-Carboxymethyl Lysine antibody [NF-1G] 画像

  • ab30917 staining Carboxymethyl Lysine in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and embedded in paraffin. Samples were incubated with primary antibody (1/125 in PBS + 1% BSA) for overnight at 4°C. A Biotin-conjugated secondary was used with DAB visualisation the samples were then counterstained with haematoxylin.

Anti-Carboxymethyl Lysine antibody [NF-1G] (ab30917) 使用論文

This product has been referenced in:
  • Crisan M  et al. Expression of Advanced Glycation End-Products on Sun-Exposed and Non-Exposed Cutaneous Sites during the Ageing Process in Humans. PLoS One 8:e75003 (2013). IHC-P ; Human . Read more (PubMed: 24116020) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - whole (cortex and hippocampus)
Specification cortex and hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RM°C
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Verified customer

投稿 Mar 28 2014

I'm sorry I was not clearer in my explanation. Both ab30917 and ab30922 should be suitable for you experiment. The reason why we sometimes have what seems to be duplicates within our catalogue is that in order to make sure we have a continuous supply o...

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Thank you for contacting us. The two antibodies (ab30917 and ab30922) are both mouse monoclonal antibodies directed against N Epsilon (Carboxymethyl) Lysine (CML). They have both been tested with ELISA, IHP (frozen sections and paraffin). They ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"