Anti-Carboxymethyl Lysine 抗体 [CML26] (ab125145)
Key features and details
- Mouse monoclonal [CML26] to Carboxymethyl Lysine
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, ELISA, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Carboxymethyl Lysine antibody [CML26]
Carboxymethyl Lysine 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CML26] to Carboxymethyl Lysine -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC/IF, ELISA, Flow Cytmore details -
種交差性
交差種: Species independent -
免疫原
Carboxymethyl Lysine conjugated to KLH.
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ポジティブ・コントロール
- Human cardiac tissue (intramyocardial arteries).
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特記事項
Other isotypes maybe present.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
CML26 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125145の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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IHC-P |
1/50.
Fix with 4% formalin. |
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IHC-Fr |
1/50.
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ICC/IF |
Use at an assay dependent concentration.
Fix with 2% phosphate-buffered glutaraldehyde solution. |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. |
IHC-P
1/50. Fix with 4% formalin. |
IHC-Fr
1/50. |
ICC/IF
Use at an assay dependent concentration. Fix with 2% phosphate-buffered glutaraldehyde solution. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy. -
別名
- CML antibody
- N Epsilon (Carboxymethyl) Lysine antibody
画像
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Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab125145 は 10 報の論文で使用されています。
- Castro B et al. SCA® Slows the Decline of Functional Parameters Associated with Senescence in Skin Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35742982
- Wang ZQ & Sun Z Dietary Nε-(carboxymethyl) lysine affects cardiac glucose metabolism and myocardial remodeling in mice. World J Diabetes 13:972-985 (2022). PubMed: 36437860
- Altomare A et al. In-Depth AGE and ALE Profiling of Human Albumin in Heart Failure: Ex Vivo Studies. Antioxidants (Basel) 10:N/A (2021). PubMed: 33673523
- Jing L et al. Role of Sortilin and Matrix Vesicles in Ne-Carboxymethyl-Lysine-Induced Diabetic Atherosclerotic Calcification. Diabetes Metab Syndr Obes 13:4141-4151 (2020). PubMed: 33177854
- Rosenstock P et al. Glycation interferes with natural killer cell function. Mech Ageing Dev 178:64-71 (2019). PubMed: 30659859
- Dubey NK et al. Adipose-derived Stem Cells Attenuates Diabetic Osteoarthritis via Inhibition of Glycation-mediated Inflammatory Cascade. Aging Dis 10:483-496 (2019). PubMed: 31164994
- Nevin C et al. Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm. Sci Rep 8:9002 (2018). PubMed: 29899461
- Frescas D et al. Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody. Proc Natl Acad Sci U S A 114:E1668-E1677 (2017). WB ; Mouse . PubMed: 28193858
- Aitken RJ et al. Involvement of homocysteine, homocysteine thiolactone, and paraoxonase type 1 (PON-1) in the etiology of defective human sperm function. Andrology 4:345-60 (2016). PubMed: 26825875
- Lo MC et al. Ne-(carboxymethyl) lysine-induced mitochondrial fission and mitophagy cause decreased insulin secretion from ß-cells. Am J Physiol Endocrinol Metab 309:E829-39 (2015). PubMed: 26394662