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RabMAb

Anti-Carbonic Anhydrase IX 抗体 [EPR4151(2)] (ab108351)

製品の概要

  • 製品名
    Anti-Carbonic Anhydrase IX antibody [EPR4151(2)]
    Carbonic Anhydrase IX 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4151(2)] to Carbonic Anhydrase IX
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the extracellular domain in Human Carbonic Anhydrase IX

  • ポジティブ・コントロール
    • HT-29 and Human stomach lysates. Human stomach and Human renal cell carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能
      Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
    • 組織特異性
      Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
    • 配列類似性
      Belongs to the alpha-carbonic anhydrase family.
      Contains 1 alpha-carbonic anhydrase domain.
    • 翻訳後修飾
      Asn-346 bears high-mannose type glycan structures.
    • 細胞内局在
      Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CA-IX antibody
      • CA9 antibody
      • CAH9_HUMAN antibody
      • CAIX antibody
      • Carbonate dehydratase IX antibody
      • Carbonic anhydrase 9 antibody
      • Carbonic anhydrase IX antibody
      • Carbonic dehydratase antibody
      • G250 antibody
      • Membrane antigen MN antibody
      • MN antibody
      • P54/58N antibody
      • pMW1 antibody
      • RCC associated protein G250 antibody
      • RCC-associated antigen G250 antibody
      • Renal cell carcinoma-associated antigen G250 antibody
      see all

    画像

    • All lanes : Anti-Carbonic Anhydrase IX antibody [EPR4151(2)] (ab108351) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : Human stomach lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab108351 at 1/100
    • Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue using ab108351 at 1/100

    参考文献

    This product has been referenced in:
    • Van den Bossche J  et al. Towards Prognostic Profiling of Non-Small Cell Lung Cancer: New Perspectives on the Relevance of Polo-Like Kinase 1 Expression, the TP53 Mutation Status and Hypoxia. J Cancer 8:1441-1452 (2017). IHC ; Human . Read more (PubMed: 28638459) »
    • Black JC  et al. Regulation of Transient Site-specific Copy Gain by MicroRNA. J Biol Chem 291:4862-71 (2016). Read more (PubMed: 26755726) »

    See all 7 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Gastric cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    Yes - Triton 0.025%
    Specification
    Gastric cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 03 2015

    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Cancer Cell Lines)
    Specification
    Cancer Cell Lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 05 2014

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Human Cell lysate - whole cell (Glioma)
    Specification
    Glioma
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 27 2013

    Application
    Western blot
    Loading amount
    30000 cells
    Gel Running Conditions
    Reduced Denaturing (11)
    Sample
    Human Cell lysate - whole cell (MCF-7 cells (human breast adenocarcinoma cell line)
    Specification
    MCF-7 cells (human breast adenocarcinoma cell line
    Treatment
    0.5% oxygen for 16hrs
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    Thank you for contacting us.

    We have several HIF antibodies in our catalog that react with mouse. For example, ab8366: HIF1 alpha, ab199: HIF2 alpha, ab10134: HIF3 alpha, ab465: HIF1 beta. If you could let me know which HIF you are looking fo...

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    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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