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Our Abpromise guarantee covers the use of ab15086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Citrate Buffer (pH 6.0)|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 25269858
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20515941Slides were fixed in acetone for 20 minutes.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840).|
Formaldehyde fixed, PBS/ 0.3% Triton X-100 permeabilized frozen human colorectal tumor tissue stained for Carbonic Anhydrase IX using ab15086 (green) at a 1/1000 dilution. 5% serum used for blocking for 1 hour at RT. Primary incubation time 3 hours at RT in PBS/ 0.3% Triton X-100/ 1% BSA. Secondary: Goat polyclonal conjugated to AlexaFluor® 488 used at a 1/2000 dilution.
ab15086 at 1/500 staining dog gastric mucosa tissue sections by IHC-P. The tissue was formaldehyde fixed and a standard citrate buffer was used for anitgen retrieval. The tissue was blocked with 10% serum prior to incubation with the antibody for 15 hours. A biotinylated donkey anti-rabbit IgG (H + L) was used as the secondary.