Anti-Carbonic Anhydrase III 抗体 - N-terminal (ab85366)

製品の概要

  • 製品名
    Anti-Carbonic Anhydrase III antibody - N-terminal
    Carbonic Anhydrase III 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Carbonic Anhydrase III - N-terminal
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the N-terminal region of Human Carbonic Anhydrase III (NP_005172), conjugated to KLH

  • ポジティブ・コントロール
    • HepG2 cell line lysate; Mouse kidney tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 30 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Reversible hydration of carbon dioxide.
  • 組織特異性
    Muscle specific.
  • 配列類似性
    Belongs to the alpha-carbonic anhydrase family.
  • 発生段階
    At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
  • 翻訳後修飾
    S-glutathionylated in hepatocytes under oxidative stress.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CA III antibody
    • CA-III antibody
    • Ca3 antibody
    • CAH3_HUMAN antibody
    • CAIII antibody
    • Car3 antibody
    • Carbonate dehydratase III antibody
    • Carbonic anhydrase 3 antibody
    • Carbonic anhydrase III antibody
    • Carbonic anhydrase III muscle specific antibody
    • EC 4.2.1.1 antibody
    • Epididymis secretory sperm binding protein Li 167mP antibody
    • HEL S 167mP antibody
    see all

Anti-Carbonic Anhydrase III antibody - N-terminal 画像

  • Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) at 1/50 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) at 1/50 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ICC/IF image of ab85366 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85366, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) 使用論文

This product has been referenced in:
  • Holland A  et al. Comparative Label-Free Mass Spectrometric Analysis of Mildly versus Severely Affected mdx Mouse Skeletal Muscles Identifies Annexin, Lamin, and Vimentin as Universal Dystrophic Markers. Molecules 20:11317-44 (2015). Read more (PubMed: 26102067) »
  • Holland A  et al. Intricate effects of primary motor neuronopathy on contractile proteins and metabolic muscle enzymes as revealed by label-free mass spectrometry. Biosci Rep 34:N/A (2014). Read more (PubMed: 24895011) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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