Anti-Carbonic Anhydrase II 抗体 [EPR5195] (ab124687)


  • 製品名Anti-Carbonic Anhydrase II antibody [EPR5195]
    Carbonic Anhydrase II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5195] to Carbonic Anhydrase II
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to a sequence from the C terminus of Human Carbonic Anhydrase II.

  • ポジティブ・コントロール
    • Human fetal brain, Caco 2, 293T, and Human heart lysates; Human kidney and brain tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値) KD = 5.42 x 10 -10 M Learn more about KD
  • バッファーpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5195
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab124687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
    • 関連疾患Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
    • 配列類似性Belongs to the alpha-carbonic anhydrase family.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CA 2 antibody
      • CA II antibody
      • CA-II antibody
      • Ca2 antibody
      • CAC antibody
      • CAH2_HUMAN antibody
      • CAII antibody
      • Car 2 antibody
      • Car2 antibody
      • Carbonate dehydratase II antibody
      • Carbonic anhydrase 2 antibody
      • Carbonic anhydrase B antibody
      • Carbonic anhydrase C antibody
      • Carbonic anhydrase C, formerly antibody
      • Carbonic anhydrase II antibody
      • Carbonic dehydratase antibody
      • epididymis luminal protein 76 antibody
      • HEL-76 antibody
      see all

    Anti-Carbonic Anhydrase II antibody [EPR5195] 画像

    • All lanes : Anti-Carbonic Anhydrase II antibody [EPR5195] (ab124687) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • ab124687, at 1/250 dilution, staining Carbonic Anhydrase II in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab124687, at 1/250 dilution, staining Carbonic Anhydrase II in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Carbonic Anhydrase II antibody [EPR5195] (ab124687) 使用論文

    ab124687 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (12.5% gel)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Treatment CA2-3xflag transfected
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

    Abcam user community

    Verified customer

    投稿 Jan 30 2015

    I heard back from the lab about the last two antibodies.
    For ab32362, the immunogen sequence shares 100% homology with pig.
    For ab124687, the immunogen peptide maps between amino acids 235 - 260, on the C-terminus of human CA2 (UniProt: P0091...

    Read More

    Thank you for your inquiry.

    For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

    Read More