Anti-Carbonic Anhydrase I 抗体 [EPR5193] (ab108367)


  • 製品名Anti-Carbonic Anhydrase I antibody [EPR5193]
    Carbonic Anhydrase I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5193] to Carbonic Anhydrase I
  • アプリケーション適用あり: WB, ICC, IHC-Frmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1

  • ポジティブ・コントロール
    • K562 and TF1 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5193
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab108367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC 1/1000 - 1/5000.
IHC-Fr 1/200.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    Anti-Carbonic Anhydrase I antibody [EPR5193] 画像

    • ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).

      See Abreview

    • All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : TF1 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 29 kDa

    Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (12.5% gel)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Treatment CA1-3xflag transfected
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

    Abcam user community

    Verified customer

    投稿 Jan 30 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Zebrafish Tissue sections (Retina, Muller cells and Horizontal cells)
    Specification Retina, Muller cells and Horizontal cells
    Permeabilization No
    Fixative Paraformaldehyde

    Dr. Ryan Macdonald

    Verified customer

    投稿 Jul 03 2013