Anti-Carbonic Anhydrase I 抗体 [EPR5193] (ab108367)

製品の概要

  • 製品名Anti-Carbonic Anhydrase I antibody [EPR5193]
    Carbonic Anhydrase I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5193] to Carbonic Anhydrase I
  • アプリケーション適用あり: WB, ICC, IHC-Frmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1

  • ポジティブ・コントロール
    • K562 and TF1 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5193
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC 1/1000 - 1/5000.
IHC-Fr 1/200.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    Anti-Carbonic Anhydrase I antibody [EPR5193] 画像

    • ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).

      See Abreview

    • All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : TF1 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa

    Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) 使用論文

    ab108367 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (12.5% gel)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Treatment CA1-3xflag transfected
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 30 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Zebrafish Tissue sections (Retina, Muller cells and Horizontal cells)
    Specification Retina, Muller cells and Horizontal cells
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Ryan MacDonald

    Verified customer

    投稿 Jul 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"