The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
関連性CAPZA3 belongs to the F actin capping protein alpha subunit family. It is predominantly localized to the neck region of ejaculated sperm, other immunohistochemical signals were found in the tail and postacrosomal regions. It may also form heterodimers of alpha and beta subunits. This protein may be important in determining sperm architecture and male fertility.