ab32787 gave a positive result in rat and mouse brain tissue lysates. CAPS1 is known not to be expressed in heart (Speidel et al, J. Biol. Chem., Vol. 278, Issue 52, 52802-52809. PMID: 14530279). Mouse heart tissue lysate can be used as a negative control for ab32787.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Can be blocked with CAPS1 peptide (ab32786). Block in 5% milk
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CAPS1 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family which consists of two members, CAPS1 and CAPS2. The CAPS family proteins are involved in the secretion of different secretory substances in developing and postnatal brains. In addition CAPS1 regulates catecholamine release from neuroendocrine cells and is expressed predominantly in the brain.
Calcium-dependent activator protein for secretion 1 antibody
Western blot - CAPS1 antibody (ab32787)
All lanes : Anti-CAPS1 antibody (ab32787) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate at 10 µg Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg Lane 3 : Heart (Mouse) Tissue Lysate at 10 µg Lane 4 : Brain (Rat) Tissue Lysate with Rat CAPS1 peptide (ab32786) at 1 µg/ml Lane 5 : Brain (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml Lane 6 : Heart (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml
Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 146 kDa Observed band size: 146 kDa
ab32787 is detecting a doublet at the expected molecular weight and both of these bands are blocked by the immunizing peptide. We are unsure what this doublet represents and would be interested in any feedback.