製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CAPS1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Rat CAPS1.

    (Peptide available as ab32786.)

  • ポジティブ・コントロール
    • ab32787 gave a positive result in rat and mouse brain tissue lysates. CAPS1 is known not to be expressed in heart (Speidel et al, J. Biol. Chem., Vol. 278, Issue 52, 52802-52809. PMID: 14530279). Mouse heart tissue lysate can be used as a negative control for ab32787.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    IHC-P: 1/300.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Can be blocked with CAPS1 peptide (ab32786). Block in 5% milk


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      CAPS1 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family which consists of two members, CAPS1 and CAPS2. The CAPS family proteins are involved in the secretion of different secretory substances in developing and postnatal brains. In addition CAPS1 regulates catecholamine release from neuroendocrine cells and is expressed predominantly in the brain.
    • 細胞内局在
      Cytoplasmic
    • 参照データベース
    • 別名
      • CADPS antibody
      • CADPS1 antibody
      • Calcium dependent secretion activator 1 antibody
      • Calcium-dependent activator protein for secretion 1 antibody
      • CAPS antibody
      • KIAA1121 antibody
      • rCAPS antibody
      see all

    Anti-CAPS1 antibody 画像

    • All lanes : Anti-CAPS1 antibody (ab32787) at 1 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate at 10 µg
      Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
      Lane 3 : Heart (Mouse) Tissue Lysate at 10 µg
      Lane 4 : Brain (Rat) Tissue Lysate with Rat CAPS1 peptide (ab32786) at 1 µg/ml
      Lane 5 : Brain (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml
      Lane 6 : Heart (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 146 kDa
      Observed band size : 146 kDa

    Anti-CAPS1 antibody (ab32787) 使用論文

    ab32787 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Dr. Sophie Pezet

    Verified customer

    投稿 Aug 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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