製品の概要

  • 製品名Anti-CAPS1 antibody
    CAPS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CAPS1
  • アプリケーション適用あり: ICC/IF, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat CAPS1.

    (Peptide available as ab32785.)

  • ポジティブ・コントロール
    • ab32011 gave a positive result in rat and mouse brain tissue lysates. CAPS1 is known not to be expressed in heart (Speidel et al, J. Biol. Chem., Vol. 278, Issue 52, 52802-52809. PMID: 14530279). Mouse heart tissue lysate can be used as a negative control for ab32011.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC-FoFr
  • 追加情報ICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Can be blocked with CAPS1 peptide (ab32785).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性CAPS1 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family which consists of two members, CAPS1 and CAPS2. The CAPS family proteins are involved in the secretion of different secretory substances in developing and postnatal brains. In addition CAPS1 regulates catecholamine release from neuroendocrine cells and is expressed predominantly in the brain.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • CADPS antibody
      • CADPS1 antibody
      • Calcium dependent secretion activator 1 antibody
      • Calcium-dependent activator protein for secretion 1 antibody
      • CAPS antibody
      • KIAA1121 antibody
      • rCAPS antibody
      see all

    Anti-CAPS1 antibody 画像

    • All lanes : Anti-CAPS1 antibody (ab32011) at 1 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate
      Lane 2 : Brain (Mouse) Tissue Lysate
      Lane 3 : Heart (Mouse) Tissue Lysate
      Lane 4 : Brain (Rat) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
      Lane 5 : Brain (Mouse) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
      Lane 6 : Heart (Mouse) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 146 kDa
      Observed band size : 146 kDa
      Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab32011 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32011, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • ab32011 staining CAPS1 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

      See Abreview

    Anti-CAPS1 antibody (ab32011) 使用論文

    ab32011 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    投稿 Jan 28 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"