製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to CAP1
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    CAP1 (NP_006358.1, 1 a.a. ~ 475 a.a) full-length human protein.

  • ポジティブ・コントロール
    • Human colon membrane lysate A-431 membrane lysate Transfected 293T membrane lysate HeLa cells Methanol treated human HeLa cells Methanol treated human promonocytic THP-1 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
  • 配列類似性
    Belongs to the CAP family.
    Contains 1 C-CAP/cofactor C-like domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenylyl cyclase associated protein 1 antibody
    • Adenylyl cyclase-associated protein 1 antibody
    • CAP 1 antibody
    • CAP antibody
    • CAP, adenylate cyclase associated protein 1 (yeast) antibody
    • CAP1 antibody
    • CAP1 PEN antibody
    • CAP1-PEN antibody
    • CAP1_HUMAN antibody
    see all

画像

  • Anti-CAP1 antibody (ab88446) at 1 µg/ml + Human colon tissue at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Anti-CAP1 antibody (ab88446) at 1 µg/ml + A-431 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • All lanes : Anti-CAP1 antibody (ab88446) at 1 µg/ml

    Lane 1 : Transfected 293T cell line
    Lane 2 : Non-transfected cell line

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunofluorescence image staining human CAP1 in HeLa cells, using ab88446 at 10µg/ml.
  • FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human HeLa cells.
  • FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human promonocytic THP-1 cells.

参考文献

ab88446 has not yet been referenced specifically in any publications.

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