Anti-Cannabinoid Receptor II 抗体 (ab45942)


  • 製品名Anti-Cannabinoid Receptor II antibody
    Cannabinoid Receptor II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cannabinoid Receptor II
  • 特異性According to BLAST results, the antibody could cross-react with both rat isoforms. No experiments were done to confirm this possibility.
  • アプリケーション適用あり: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Cannabinoid Receptor II.

    (Peptide available as ab45941.)

  • ポジティブ・コントロール
    • ab45942 gave a positive result in the following tissue lysates: Rat Spinal Cord, and Mouse Thymus.
  • 特記事項

    Please note that this antibody is not suitable for the use with human samples.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab45942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).Can be blocked with Cannabinoid Receptor II peptide (ab45941).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.


  • 機能Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • 組織特異性Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • 細胞内局在Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

Anti-Cannabinoid Receptor II antibody 画像

  • Immunocytochemical immunofluorescence analysis of methanol-fixed HT29 human cell line, labelling cannabinoid receptor II with ab45942 at 1/50 dilution incubated for 18 hours at 4°C in 1% BSA. Fixed cells were permeabilized with 0.25% Tween. Secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 488. Counterstain is DAPI against nuclear DNA.

    See Abreview

  • All lanes : Anti-Cannabinoid Receptor II antibody (ab45942) at 1 µg/ml

    Lane 1 : Rat spinal cord tissue lysate
    Lane 2 : Mouse thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

    Exposure time : 8 minutes
  • ICC/IF image of ab45942 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45942, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cannabinoid Receptor II antibody (ab45942) 使用論文

This product has been referenced in:
  • den Boon FS  et al. Excitability of prefrontal cortical pyramidal neurons is modulated by activation of intracellular type-2 cannabinoid receptors. Proc Natl Acad Sci U S A 109:3534-9 (2012). WB ; Rat . Read more (PubMed: 22331871) »
  • Tyagi V  et al. Differential expression of functional cannabinoid receptors in human bladder detrusor and urothelium. J Urol 181:1932-8 (2009). WB, IHC-Fr ; Human . Read more (PubMed: 19237176) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT29 cell line (colon cancer epithelial cell line))
Permeabilization Yes - Tween 0,25%
Specification HT29 cell line (colon cancer epithelial cell line)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Methanol

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投稿 Oct 30 2015

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 20 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jun 01 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

投稿 Apr 25 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

投稿 May 26 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

投稿 Oct 01 2008