Anti-Cannabinoid Receptor II 抗体 (ab3560)

製品の概要

  • 製品名
    Anti-Cannabinoid Receptor II antibody
    Cannabinoid Receptor II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cannabinoid Receptor II
  • アプリケーション
    適用あり: ICC/IF, IP, IHC-Fr, Flow Cyt, IHC-P, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Hamster, Human, Non human primates
  • 免疫原

    Fusion protein corresponding to Human Cannabinoid Receptor II aa 1-33 (N terminal).

  • ポジティブ・コントロール
    • Human spinal cord lysate IF/ICC: SKNSH cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 22387618
IHC-Fr 1/100.
Flow Cyt 1/50.
IHC-P 1/100.
ICC 1/100.
WB 1/250 - 1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 機能
    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • 組織特異性
    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾
    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • 細胞内局在
    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

画像

  • ICC/IF image of ab3560 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3560, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Kleyer J  et al. Cannabinoid receptor trafficking in peripheral cells is dynamically regulated by a binary biochemical switch. Biochem Pharmacol 83:1393-412 (2012). Flow Cyt, IP ; Human . Read more (PubMed: 22387618) »

See 1 Publication for this product

レビューと Q&A

Application
ELISA
Sample
Human Recombinant protein (H00001269-P01 (Novusbio), CNR2 recombinant protein)
Specification
H00001269-P01 (Novusbio), CNR2 recombinant protein
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Indirect
Username

Miss. Carina Kozmus

Verified customer

投稿 Nov 25 2015

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
No
Gating Strategy
Lymphocytes B (CD19+)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: ficoll
Sample buffer: PBS 1%BSA 0.01%NaN3
Username

Abcam user community

Verified customer

投稿 Oct 16 2015

Thank you for contacting us.

We have following CB1 and CB2 available. These are testes in flow cytometry and are fully guaranteed.

ab3558; http://www.abcam.com/Cannabinoid-Receptor-I-antibody-ab3558.html

ab3561; http://www.ab...

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Thank you for your inquiry.

The best way to find isotype controls for an antibody is to start at our homepage www.abcam.com. Go to the yellow bar at the top of the page and move the mouse to “Products,” then click on “Iso...

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This product has been successfully tested in IHC with frozen sections. We have not tested the product with paraffin sections (formalin-fixed). It may work, but I would definitely use an antigen retrieval step. We have protocols listed on our website...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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