The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa). 65kDa band detected in rat brain lysate. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343).
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
Belongs to the G-protein coupled receptor 1 family.
Kim J et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol5:100 (2014).
Read more (PubMed: 24711795) »