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We have developed detailed protocols that include :
The protocols are available to download for rapid implementation in your experiment to get the best results.
Automated platforms with validated protocols:
The panel aboves shows staining using anti PD-L1 [28-8] RabMAb® antibody (ab205921) on wild type L2987 cells (Left) and PD-L1 knockout (KO) line of L2987 (right). PD-L1 gene was edited in L2987 colorectal cancer cell lines using TALEN constructs targeting exon 4 of human PD-L1. Complete KO was confirmed by deep sequencing.
To support your specific experiment, we have developed a wide range of reagents using our PD-L1 [clone 28-8] to suit your experiments.
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.