製品の概要

  • 製品名Anti-CAMLG antibody
    CAMLG 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to CAMLG
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human CAMLG protein

  • ポジティブ・コントロール
    • Transfected 293T cell lysate; rat brain tissue lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 20676093
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.

ターゲット情報

  • 関連性The immunosuppressant drug cyclosporin A blocks a calcium dependent signal from the T cell receptor (TCR) that normally leads to T cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. [Entrez Gene]
  • 細胞内局在Membrane; Multi pass membrane protein.
  • 参照データベース
  • 別名
    • calcium modulating cyclophilin ligand antibody
    • calcium modulating ligand antibody
    • Calcium signal modulating cyclophilin ligand antibody
    • CAML antibody
    • cyclophilin B binding protein antibody
    • MGC163197 antibody
    see all

Anti-CAMLG antibody 画像

  • Anti-CAMLG antibody (ab67714) at 1/500 dilution + rat brain at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • All lanes : Anti-CAMLG antibody (ab67714) at 1/500 dilution

    Lane 1 : CAMLG transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • ICC/IF image of ab67714 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67714, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CAMLG antibody (ab67714) 使用論文

This product has been referenced in:
  • He B  et al. The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88. Nat Immunol 11:836-45 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20676093) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab67714.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"