Anti-CAMKK2 抗体 (ab96531)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CAMKK2
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 1-15 of CAMKK2 isoform 5 (NP_705720)

  • ポジティブ・コントロール
    • H1299 Whole cell lysate or HeLaS3 cell lysates. HeLa cells (IF)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
ICC/IF 1/100 - 1/200.
IHC-FoFr 1/1000.

ターゲット情報

  • 機能
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.
  • 組織特異性
    Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • ドメイン
    The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
    The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium/calmodulin dependent protein kinase beta antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
    • Calcium/calmodulin dependent protein kinase kinase beta antibody
    • Calcium/calmodulin-dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin-dependent protein kinase kinase beta antibody
    • CaM kinase kinase beta antibody
    • CaM KK beta antibody
    • CaM-kinase kinase 2 antibody
    • CaM-kinase kinase beta antibody
    • CaM-KK 2 antibody
    • CaM-KK beta antibody
    • CaMKK 2 antibody
    • CAMKK antibody
    • CaMKK beta antibody
    • CAMKK beta protein antibody
    • Camkk2 antibody
    • CAMKKB antibody
    • KKCC2_HUMAN antibody
    see all

画像

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa using ab96531 at 1/200 dilution: (1) without DNA probe; (2) merged with DNA probe.
  • Anti-CAMKK2 antibody (ab96531) at 1/500 dilution (10% SDS PAGE) + H1299 whole cell lysate at 30 µg

    Predicted band size : 55 kDa
  • IHC-FoFr image of CAMKK2 using ab96531 staining rat spinal cord.The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

参考文献

ab96531 has not yet been referenced specifically in any publications.

レビューと Q&A

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I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have...

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Thank you for contacting us.

I have found the amino acid sequence for LKB1 for Dictyostelium which can be found at http://www.uniprot.org/uniprot/Q15831.fasta

I have contacted the lab to see if any of our antibodies would be compat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Karine Thibault

Verified customer

投稿 Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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