製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CAMKK2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within amino acids 42-71 of Mouse CAMKK2 (NP_663333.1).

  • ポジティブ・コントロール
    • NIH 3T3 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.

ターゲット情報

  • 機能
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.
  • 組織特異性
    Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • ドメイン
    The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
    The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium/calmodulin dependent protein kinase beta antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
    • Calcium/calmodulin dependent protein kinase kinase beta antibody
    • Calcium/calmodulin-dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin-dependent protein kinase kinase beta antibody
    • CaM kinase kinase beta antibody
    • CaM KK beta antibody
    • CaM-kinase kinase 2 antibody
    • CaM-kinase kinase beta antibody
    • CaM-KK 2 antibody
    • CaM-KK beta antibody
    • CaMKK 2 antibody
    • CAMKK antibody
    • CaMKK beta antibody
    • CAMKK beta protein antibody
    • Camkk2 antibody
    • CAMKKB antibody
    • KKCC2_HUMAN antibody
    see all

画像

  • Anti-CAMKK2 antibody (ab124096) at 1/100 dilution + NIH 3T3 cell lysate at 35 µg

    Predicted band size: 65 kDa

プロトコール

参考文献

This product has been referenced in:
  • Sun Q  et al. Imidacloprid Promotes High Fat Diet-Induced Adiposity in Female C57BL/6J Mice and Enhances Adipogenesis in 3T3-L1 Adipocytes via the AMPKa-Mediated Pathway. J Agric Food Chem 65:6572-6581 (2017). Read more (PubMed: 28704996) »

See 1 Publication for this product

レビューと Q&A

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mice whole cell)
Total protein in input
50 µg
Immuno-precipitation step
Protein G
Specification
mice whole cell
Username

Valiya Anuradha

Verified customer

投稿 Apr 03 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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