製品の概要

  • 製品名
    Anti-CAMKIV antibody [EP2564Y]
    CAMKIV 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2564Y] to CAMKIV
  • アプリケーション
    適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the N terminal region of human CaMKIV.

  • ポジティブ・コントロール
    • Jurkat cell lysate and human brain lysate.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能
      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • 組織特異性
      Expressed in epithelial ovarian cancer tissue.
    • 配列類似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • 細胞内局在
      Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
      • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
      • Brain Ca++-calmodulin dependent protein kinase type IV antibody
      • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
      • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
      • Calcium/calmodulin dependent protein kinase IV antibody
      • Calcium/calmodulin dependent protein kinase type IV antibody
      • Calcium/calmodulin-dependent protein kinase type IV antibody
      • CAM kinase 4 antibody
      • CAM kinase GR antibody
      • CAM kinase IV antibody
      • CAM kinase-GR antibody
      • CaMK 4 antibody
      • CAMK GR antibody
      • CaMK IV antibody
      • Camk4 antibody
      • CaMKGR antibody
      • IV antibody
      • KCC4_HUMAN antibody
      • MGC36771 antibody
      see all

    画像

    • All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 52 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27611779) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for your reply.
    Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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    Thank you very much for your interest in our antibodies.

    We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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