製品の概要

  • 製品名Anti-CamKII gamma antibody
    CamKII gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CamKII gamma
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human CamKII gamma aa 309-338 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • SKBR3 cell lysate, mouse heart tissue lysate, human hepatocarcinoma This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

Anti-CamKII gamma antibody 画像

  • ICC/IF image of ab37999 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37999 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CamKII gamma antibody (ab37999) at 1/1000 dilution + mouse heart tissue lysate at 35 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 88 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37999 at 1/100 dilution staining CamKII in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by AEC staining.

Anti-CamKII gamma antibody (ab37999) 使用論文

This product has been referenced in:
  • Chakraborty A  et al. Inhibition of CaMKII does not attenuate cardiac hypertrophy in mice with dysfunctional ryanodine receptor. PLoS One 9:e104338 (2014). WB ; Mouse . Read more (PubMed: 25093823) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab37999.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"