製品の概要

  • 製品名Anti-CaMKII beta antibody
    CaMKII beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CaMKII beta
  • 特異性This antibody does cross-react with CAMKII alpha protein
  • アプリケーション適用あり: WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Recombinant fragment: FEPEALGNLV EGMDFHRFYF ENLLAKNSKP IHTTILNPHV HVIGEDAACI AYIRLTQYID GQGRPRTSQS EETRVWHRRD GKWQNVHFHC SGAPVAPL, corresponding to amino acids 405-502 of human CaMKII beta (NP_742078) with a proprietary tag.

  • ポジティブ・コントロール
    • WB: The immunogen; HeLa and PC12 cell lysates ICC/If: HeLa cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.

Predicted molecular weight: 73 kDa for isoform 1, and 56 kDa for isoform 2.

ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 25 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • 組織特異性Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 製品の状態The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
  • 別名
    • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase IIB antibody
    • Calcium/calmodulin dependent protein kinase type II beta chain antibody
    • calcium/calmodulin-dependent protein kinase II beta antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
    • CAM 2 antibody
    • CaM kinase II beta chain antibody
    • CaM kinase II beta subunit antibody
    • CaM kinase II subunit beta antibody
    • CaM-kinase II beta chain antibody
    • CAM2 antibody
    • CAMK 2 antibody
    • CAMK 2B antibody
    • CaMK II beta subunit antibody
    • CaMK II subunit beta antibody
    • CaMK-II subunit beta antibody
    • CAMK2 antibody
    • CaMK2 beta antibody
    • Camk2b antibody
    • CAMKB antibody
    • CaMKII beta subunit antibody
    • CaMKIIB antibody
    • KCC2B_HUMAN antibody
    • MGC29528 antibody
    • Proline rich calmodulin dependent protein kinase antibody
    • proline rich calmodulin-dependent protein kinase antibody
    see all

Anti-CaMKII beta antibody 画像

  • Overlay histogram showing SH-SY5Y cells stained with ab89197 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89197, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-CaMKII beta antibody (ab89197) at 5 µg/ml + tagged, recombinant human CaMKII beta protein fragment (the immunogen) at 0.2 µg

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-CaMKII beta antibody (ab89197) at 5 µg/ml + HeLa cell lysate at 50 µg

    Observed band size : ~48 kDa (why is the actual band size different from the predicted?)
  • Anti-CaMKII beta antibody (ab89197) at 5 µg/ml + PC12 cell lysate at 50 µg

    Observed band size : ~47 kDa (why is the actual band size different from the predicted?)
  • ab89197, at 25µg/ml, staining CaMKII beta in HeLa cell by Immunofluorescence.

Anti-CaMKII beta antibody (ab89197) 使用論文

This product has been referenced in:
  • Buckley PT  et al. Cytoplasmic Intron Sequence-Retaining Transcripts Can Be Dendritically Targeted via ID Element Retrotransposons. Neuron 69:877-84 (2011). ICC/IF ; Rat . Read more (PubMed: 21382548) »

See 1 Publication for this product

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Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"