製品の概要
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製品名Anti-CaMKII beta antibody
CaMKII beta 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CaMKII beta
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由来種Rabbit
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特異性This antibody does cross-react with CAMKII alpha protein
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アプリケーション適用あり: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
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種交差性交差種: Mouse, Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse CaMKII beta.
(Peptide available as ab27843.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue Brain (Rat) Tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab34703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | 1/300. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Mouse CaMKII beta peptide (ab27843). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
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組織特異性Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
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配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 12323 Mouse
- Entrez Gene: 24245 Rat
- SwissProt: P28652 Mouse
- SwissProt: P08413 Rat
- Unigene: 439733 Mouse
- Unigene: 9743 Rat
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製品の状態The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
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別名
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase IIB antibody
see all
画像
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Western blot - CaMKII beta antibody (ab34703)All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml
Lane 1 : Brain Mouse Tissue Lysate
Lane 2 : Brain Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Immunohistochemistry (PFA perfusion fixed frozen sections) - CaMKII beta antibody (ab34703)This image is courtesy of an Abreview submitted by Dr Sophie Pezetab34703 staining CaMKII in rat brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgGwas used as secondary at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody (ab34703)Image courtesy of an anonymous Abreview.ab34703 staining CaMKII beta in adult mouse cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 0.01M Tris HCL, pH 10. Samples were then permeabilized using 0.3% Triton X-100, blocked with 5% BSA for 45 minutes at 22°C and then incubated with ab34703 at a 1/3000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/2000 dilution.
Strong siganls were detected in molecular layers (M), and Purkinje cells (P) were positively stained as well. In granule layers (G), some neurons were also strongly stained. -
Immunocytochemistry/ Immunofluorescence - Anti-CaMKII beta antibody (ab34703)ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Li Y et al. Role of the Lateral Habenula in Pain-Associated Depression. Front Behav Neurosci 11:31 (2017). WB . Read more (PubMed: 28270756) »
- Yu L et al. Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+Signaling In Vitro. Biomed Res Int 2017:1404650 (2017). Read more (PubMed: 29164146) »
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