Anti-CaMKII alpha + CaMKII beta (phospho T286) 抗体 (ab91290)

製品の概要

  • 製品名
    Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody
    CaMKII alpha + CaMKII beta (phospho T286) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CaMKII alpha + CaMKII beta (phospho T286)
  • 特異性
    Due to the homology between the immunogen peptide and other CAMKII isoforms, ab124880 may cross react with CAMKII delta (KCC2D, 100% homology)
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Rabbit, Chicken, Cow, Human, Pig, Orangutan
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CaMKII alpha + CaMKII beta (phospho T286) aa 250-350 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab155646, ab115237, ab115237)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in rat brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 関連性
    Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
  • 参照データベース
  • 別名
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
    • Calcium/calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase IIB antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin dependent protein kinase type II beta chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II antibody
    • calcium/calmodulin-dependent protein kinase II beta antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CAM 2 antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II antibody
    • CaM kinase II beta chain antibody
    • CaM kinase II beta subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaM kinase II subunit beta antibody
    • CaM-kinase II beta chain antibody
    • CAM2 antibody
    • CAMK 2 antibody
    • CaMK II alpha subunit antibody
    • CaMK II beta subunit antibody
    • CaMK-II antibody
    • CaMK-II subunit alpha antibody
    • CaMK-II subunit beta antibody
    • Camk2 antibody
    • Camk2a antibody
    • Camk2b antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • KCC2_HUMAN antibody
    • KCC2A_HUMAN antibody
    • KCC2B_HUMAN antibody
    see all

画像

  • All lanes : Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody (ab91290) at 1/250 dilution

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate with CaMKII alpha (phospho T286) peptide (ab115237) at 1 µg/ml
    Lane 3 : Brain (Rat) Tissue Lysate with Human CaMKII alpha peptide (ab155646) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

ab91290 has not yet been referenced specifically in any publications.

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