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製品の概要

  • 製品名Anti-CaMKII alpha antibody [EPR1828]
    CaMKII alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1828] to CaMKII alpha
  • 特異性The peptide immunogen shares a 100% homology with CaMKII beta, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
  • アプリケーション適用あり: WB, IP, IHC (PFA fixed), Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminal of Human CaMKII alpha.

  • ポジティブ･コントロール
    • Rat brain lysate, SH SY5Y cell lysate, Neuro 2a cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IP 1/10 - 1/100.
IHC (PFA fixed) Use at an assay dependent concentration. PubMed: 24866701
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC 2.7.11.17 antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

Anti-CaMKII alpha antibody [EPR1828] 画像

  • Flow Cytometry - Anti-CaMKII alpha antibody [EPR1828] (ab92332)
    Flow Cytometry - Anti-CaMKII alpha antibody [EPR1828] (ab92332)
    Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - CaMKII alpha antibody [EPR1828] (ab92332)
    Western blot - CaMKII alpha antibody [EPR1828] (ab92332)
    All lanes : Anti-CaMKII alpha antibody [EPR1828] (ab92332)

    Lane 1 : Rat brain lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : Neuro 2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 54 kDa

Anti-CaMKII alpha antibody [EPR1828] (ab92332) 使用論文

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See 1 Publication for this product

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ab92332 の abreview を投稿する ab92332 に関する問い合わせをする

1 Abreview or Q&A

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Votes: Highest First

Hi, I am interested in ELISA kit for the human CAMKII alpha (ab2724) . There is no ready to use kit and I wonder if you can help me to choose anitbodies for ELISA sandwich. I have already use the two of your products for Western Blot. ab22...

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Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"