リコンビナント
Anti-CaMKII alpha 抗体 [EPR1828] (ab92332)
製品の概要
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製品名Anti-CaMKII alpha antibody [EPR1828]
CaMKII alpha 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR1828] to CaMKII alpha
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特異性The peptide immunogen shares a 100% homology with CaMKII beta, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
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アプリケーション適用あり: WB, IP, IHC (PFA fixed), Flow Cytmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide corresponding to residues near the N terminal of Human CaMKII alpha.
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ポジティブ･コントロール
- Rat brain lysate, SH SY5Y cell lysate, Neuro 2a cell lysate
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特記事項
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR1828
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab92332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 54 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC (PFA fixed) | Use at an assay dependent concentration. PubMed: 24866701 | |
| Flow Cyt | 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
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配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
細胞内局在Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
- Information by UniProt
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参照データベース
- Entrez Gene: 815 Human
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- Omim: 114078 Human
- SwissProt: Q9UQM7 Human
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
- Unigene: 725992 Human
see all -
別名
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
Anti-CaMKII alpha antibody [EPR1828] 画像
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![Flow Cytometry - Anti-CaMKII alpha antibody [EPR1828] (ab92332)](http://a.abcam.com/ps/products/92/ab92332/Images/ab92332-2-ab92332FC.jpg)
Flow Cytometry - Anti-CaMKII alpha antibody [EPR1828] (ab92332)Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - CaMKII alpha antibody [EPR1828] (ab92332)](http://a.abcam.com/ps/datasheet/images/92/ab92332/CaMKII-alpha-Primary-antibodies-ab92332-1.jpg)
Western blot - CaMKII alpha antibody [EPR1828] (ab92332)All lanes : Anti-CaMKII alpha antibody [EPR1828] (ab92332)
Lane 1 : Rat brain lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Neuro 2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 54 kDa
プロトコール
Anti-CaMKII alpha antibody [EPR1828] (ab92332) 使用論文
This product has been referenced in:
- Xu Z et al. Entorhinal Principal Neurons Mediate Brain-stimulation Treatments for Epilepsy. EBioMedicine 14:148-160 (2016). IHC-P ; Mouse . Read more (PubMed: 27908611) »
- La Montanara P et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem 290:4512-27 (2015). Read more (PubMed: 25555910) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"