製品の概要

  • 製品名Anti-CaMKII alpha antibody
    CaMKII alpha 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CaMKII alpha
  • 特異性Expected to recognize both reported isoforms of CaMKII (NP_057065.2 and NP_741960.1). This antibody does cross-react with CAMKII beta protein
  • アプリケーション適用あり: IHC-P, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Peptide with sequence

    C-PRTAQSEETRVWHR

    , corresponding to internal sequence amino acids 445-458 of human CaMKII alpha protein (NP_057065.2; NP_741960.1). (Peptide available as ab126462.)

  • ポジティブ・コントロール
    • Human brain (cerebellum) and mouse brain lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human CaMKII alpha peptide (ab126462).
IHC-FoFr Use at an assay dependent concentration. PubMed: 24231734

ターゲット情報

  • 機能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC 2.7.11.17 antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

Anti-CaMKII alpha antibody 画像

  • Anti-CaMKII alpha antibody (ab87597) at 0.1 µg/ml + mouse brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab87597 at 3.8 µg/ml staining CaMKII in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

Anti-CaMKII alpha antibody (ab87597) 使用論文

This product has been referenced in:
  • Gan JO  et al. Adolescent social isolation enhances the plasmalemmal density of NMDA NR1 subunits in dendritic spines of principal neurons in the basolateral amygdala of adult mice. Neuroscience 258:174-83 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24231734) »
  • Zhong W  et al. Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva). PLoS One 9:e104718 (2014). IHC-Fr . Read more (PubMed: 25121483) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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投稿 Dec 30 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Cat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 13 2012

I am very pleased to hear you would like to accept our offer and test ab87597 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in th...

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Thank you very much for your interest in ab87597.

To our knowledge, ab87597 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab87597 now, test it in IHC-Fr and submit feedback to us in the f...

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Vielen Dank für Ihre Anfrage.

Ich kann empfehlen den ab87597 mit einer Verdünnungreihe, ausgehend von 1/100 )zum Beispiel 1/50, 1/100 und 1/200) auszutested. Auch kann ich empfehlen, die Antigendemaskierung mit einem Citrate Puffer pH 6 unte...

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Thank you for contacting Abcam yesterday.

I have talked to the lab and we will be able to get the blocking peptide for ab87597 added to our catalogue. The product should be added in a day or so, therefore you should be able to purchase it by ...

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