製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CaMKI gamma
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.

    (Peptide available as ab38755.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
  • 組織特異性
    Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • ドメイン
    The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • 翻訳後修飾
    May be prenylated on Cys-473.
  • 細胞内局在
    Cytoplasm. Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium/calmodulin dependent protein kinase type 1G antibody
    • Calcium/calmodulin-dependent protein kinase I-gamma antibody
    • Calcium/calmodulin-dependent protein kinase Ig antibody
    • Calcium/calmodulin-dependent protein kinase type 1G antibody
    • CaM kinase I gamma antibody
    • CaM kinase IG antibody
    • CaM-KI gamma antibody
    • CaMK like CREB kinase III antibody
    • CaMK-like CREB kinase III antibody
    • Camk1g antibody
    • CaMKI gamma antibody
    • CaMKIG antibody
    • CLICK III antibody
    • CLICK3 antibody
    • dJ272L16.1 antibody
    • KCC1G_HUMAN antibody
    • VWS1 antibody
    see all

画像

  • Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 79 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

参考文献

ab38132 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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