製品の概要

  • 製品名Anti-CaMKI antibody [EP2218Y]
    CaMKI 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2218Y] to CaMKI
  • アプリケーション適用あり: WB, IP, ICC, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human CaMK1

  • ポジティブ・コントロール
    • SH-SY5Y cell lysate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP 1/20.
ICC 1/100 - 1/250.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
    • 組織特異性Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
    • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • ドメインThe autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
    • 翻訳後修飾Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
      Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
    • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Calcium / calmodulin dependent protein kinase 1 antibody
      • Calcium / calmodulin dependent protein kinase I antibody
      • Calcium / calmodulin dependent protein kinase type 1 antibody
      • Calcium/calmodulin dependent protein kinase 1 antibody
      • Calcium/calmodulin dependent protein kinase I antibody
      • Calcium/calmodulin dependent protein kinase type 1 antibody
      • Calcium/calmodulin-dependent protein kinase type 1 antibody
      • CaM K1 antibody
      • CaM KI antibody
      • CaM kinase 1 alpha antibody
      • CAM kinase 1 antibody
      • CaM kinase I alpha antibody
      • CaM kinase I antibody
      • CaM-KI antibody
      • CaMK 1 antibody
      • CAMK I antibody
      • CaMK1 alpha antibody
      • CAMK1 antibody
      • CAMK1 PEN antibody
      • CaMKI alpha antibody
      • CaMKI-alpha antibody
      • KCC1A_HUMAN antibody
      • MGC120317 antibody
      • MGC120318 antibody
      see all

    Anti-CaMKI antibody [EP2218Y] 画像

    • Anti-CaMKI antibody [EP2218Y] (ab62374) at 1/20000 dilution + SH-SY5Y cell lysate at 10 µg

      Secondary
      goat anti-rabbit, HRP labeled, at 1/2000 dilution

      Predicted band size : 41 kDa
      Observed band size : 41 kDa

    Anti-CaMKI antibody [EP2218Y] (ab62374) 使用論文

    This product has been referenced in:
    • Pfisterer SG  et al. Ca2+/calmodulin-dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 at the onset of autophagy. Mol Pharmacol 80:1066-75 (2011). WB ; Human . Read more (PubMed: 21896713) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7)
    Loading amount 10000 cells
    Specification huh-7
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Jun 26 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"