製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Calumenin
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Cow, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Calumenin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human tissue lysates: Placenta; Skeletal muscle; Lung.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 機能
    Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
  • 組織特異性
    Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
  • 配列類似性
    Belongs to the CREC family.
    Contains 6 EF-hand domains.
  • 細胞内局在
    Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CALU antibody
    • CALU_HUMAN antibody
    • Calumenin 1 antibody
    • Calumenin 2 antibody
    • Calumenin antibody
    • Crocalbin antibody
    • crocalbin-like protein antibody
    • FLJ90608 antibody
    • IEF SSP 9302 antibody
    • multiple EF-hand protein antibody
    • OTTHUMP00000212712 antibody
    • OTTHUMP00000212713 antibody
    • OTTHUMP00000212714 antibody
    • OTTHUMP00000212715 antibody
    see all

画像

  • All lanes : Anti-Calumenin antibody (ab102115) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunising peptide at 1 µg/ml
    Lane 6 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa,30 kDa,42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

ab102115 has not yet been referenced specifically in any publications.

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