製品の概要

  • 製品名Anti-Calretinin antibody [SP13]
    Calretinin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP13] to Calretinin
  • 特異性Ab16694 recognises Calretinin.
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant full length calretinin protein (Mouse).

  • ポジティブ・コントロール
    • Human mesothelioma for Immunohistochemistry.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide.
    Constituents: Tissue culture supernatant.
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名SP13
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.

ターゲット情報

Anti-Calretinin antibody [SP13] 画像

  • Immunocytochemical analysis of 5% PFA & 0.2% Picric Acid fixed rat cordical cell sections, labelling Calretinin with ab16694 at a dilution of 1/10 incubated fro 12 hours at 4°C in a 10mM PBS & 0.35% Triton X diluent. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.

    See Abreview

  • Ab16694 at a dilution of 1/100, staining Calretinin in formalin fixed paraffin embedded human mesothelioma tissue section by Immunohistochemistry.

Anti-Calretinin antibody [SP13] (ab16694) 使用論文

This product has been referenced in:
  • Kuwabara T  et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci 12:1097-105 (2009). ICC/IF ; Rat . Read more (PubMed: 19701198) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat Cortical Cell Culture)
Permeabilization No
Specification Rat Cortical Cell Culture
Fixative 5% PF, 02% Picric Acid
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Ms. Babben Tinner

Verified customer

投稿 Oct 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"