製品の概要

  • 製品名Anti-Calretinin antibody
    Calretinin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Calretinin
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cat, Dog, Human, Xenopus laevis, Zebrafish
  • 免疫原

    Full length protein

  • ポジティブ・コントロール
    • Mesothelioma.
  • 特記事項Contains a proprietary preservative that is not sodium azide or thimerosal.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, Carrier protein, Van Gogh Yellow Diluent, pH 7.3
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • ミエローマunknown
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.
IHC-Fr 1/100. PubMed: 20504962

Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).

ターゲット情報

Anti-Calretinin antibody 画像

  • ab702 staining Calretinin in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab702 staining Calretinin (green) in zebrafish retinal cells by Immunohistochemistry (frozen sections).
    Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat.

    Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped.

    Original article: http://dev.biologists.org/content/137/13/2107.long
  • Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.

Anti-Calretinin antibody (ab702) 使用論文

This product has been referenced in:
  • Taylor SR  et al. Neuroblast Distribution after Cortical Impact Is Influenced by White Matter Injury in the Immature Gyrencephalic Brain. Front Neurosci 10:387 (2016). Read more (PubMed: 27601978) »
  • Fatt MP  et al. p63 and p73 coordinate p53 function to determine the balance between survival, cell death, and senescence in adult neural precursor cells. Cell Death Differ N/A:N/A (2014). Read more (PubMed: 24809925) »

See all 15 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample Mouse Tissue sections (frozen brain section)
Specification frozen brain section
Permeabilization No
Fixative Paraformaldehyde
Username

Mrs. Bettina Hoerz

Verified customer

投稿 Jan 06 2014



We do not have specific use by dates for our products.

If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

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Gracias por tu respuesta.

Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas ...

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Gracias por contactarnos.


Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuaci...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"