Anti-Calreticulin 抗体 [EPR3924] - ER Marker (ab92516)



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR3924
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab92516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of a HRP/AP polymerized secondary antibody is recommended for enhanced staining.

Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.


  • 機能Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 配列類似性Belongs to the calreticulin family.
  • ドメインCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 細胞内局在Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Anti-Calreticulin antibody [EPR3924] - ER Marker 画像

  • Predicted band size : 48 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Calreticulin knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: NIH3T3 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92516 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab92516 was shown to specifically react with Calreticulin when Calreticulin knockout samples were used. Wild-type and Calreticulin knockout samples were subjected to SDS-PAGE. ab92516 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling Calreticulin with purified ab92516 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • ab92516 showing positive staining in Normal colon tissue.

  • Overlay histogram showing HeLa cells stained with ab92516 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92516, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HL-60 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 48 kDa
  • ab92516, at 1/250 dilution, staining Calreticulin in paraffin embedded Human kidney tissue by Immunohistochemistry.
  • ab92516 showing negative staining in Normal heart tissue.

  • ab92516 showing positive staining in Normal liver tissue.

  • ab92516 showing positive staining in Normal placenta tissue.

  • ab92516 showing positive staining in Normal stomach tissue.

  • ab92516 showing positive staining in Papillary carcinoma of thyroid gland tissue.

Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516) 使用論文

This product has been referenced in:
  • Klein J  et al. The KUPKB: a novel Web application to access multiomics data on kidney disease. FASEB J 26:2145-53 (2012). ICC/IF ; Human, Rat . Read more (PubMed: 22345404) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Sample Sacrophilus harrisii (Tasmanian Devil) Cell (C5065 (tasmanian devil tumour cell line))
Permeabilization Yes - PBS/0.1% tween
Gating Strategy Population was gated for single cells and DAPI used to exclude dead cells.
Specification C5065 (tasmanian devil tumour cell line)
Preparation Cell harvesting/tissue preparation method: Cells were washed in PBS counted and resuspended at 5x10/6 cells per ml in FACS buffer. 100ul was added to tubes for fixation, permeabilisation and staining.
Sample buffer: FACS buffer (PBS/0.5% BSA/0.05% azide)
Fixation 80% methanol

Abcam user community

Verified customer

投稿 Dec 01 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step 3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Monkey Cell (Kidney)
Specification Kidney
Permeabilization No
Fixative 3.2% PFA and 0.5% GA at 37C

Dr. Aaron Halpen

Verified customer

投稿 Sep 23 2014

Thank you for contacting Abcam regarding ab92516. Regarding Flow cytometry, I have confirmed that we used an intracellular cell staining protocol as we reviewed on the phone.  The lab has indicated that the cells need to be fixed with 2% paraformal...

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